Gene classification

Compared to ZS11 RefSeq, pan-genome added 781.9 Mb DNA sequence and 21,020 coding genes. All genes were classified into core genes and distribution genes by their presence in each variety. Further distributed genes are further divided into subspecies imbalance genes (frequency in one subspecies is significantly higher than that in other subspecies, P value <0.05), subspecies specific genes (>95% in one subspecies) and random genes, according to the frequency of existence of genes in different subspecies. Breeders were supposed to focus on the present accessions in selecting breeding donors.